Transfection Reagents
SignaGen Laboratories GENJET DNA REAGENT B16 1ML
GenJet DNA In Vitro Tranfection Reagent for B16 was pre-optimized for most efficiently transfecting B16 cells.
SignaGen Laboratories GENJET DNA REAGENT PAC 1ML
GenJet DNA In Vitro Tranfection Reagent for PAC was pre-optimized for most efficiently transfecting PAC cells.
SignaGen Laboratories GENJET DNA REAGENT CV1 1ML
GenJet DNA In Vitro Tranfection Reagent for CV1 was pre-optimized for most efficiently transfecting CV1 cells.
Supply Solutions Roche - X-tremeGENE HP DNA Transf. Reag. 1.0 ml; High-performance polymer reagent for transfecting many cell lines; pkg of 0.4 mL (06366244001) pkg of 1.0mL (06366236001) pkg of 5 x 1 mL (06366546001)
Roche - X-tremeGENE HP DNA Transf. Reag. 1.0 ml; High-performance polymer reagent for transfecting many cell lines; pkg of 0.4 mL (06366244001) pkg of 1.0mL (06366236001) pkg of 5 x 1 mL (06366546001)
Lonza Walkersville Human T Cell Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human T cells (unstimulated or pre-stimulated).The cell type-specific Optimized Protocol is available in our knowledge database. In this Optimized Protocols the best Nucleofection Conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human T Cells0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL), 25 Single use pipettes
STEMCELL Technologies Resazurin (Sodium Salt), 5 g
ReproRNA™-OKSGM is a single-stranded RNA replicon vector that contains five reprogramming factors: OCT4, KLF-4, SOX2, GLIS1, and c-MYC, as well as a puromycin-resistance gene (Figure 1). This RNA vector reprograms somatic cells, such as fibroblasts, into induced pluripotent stem (iPS) cells with high efficiency and only requires a single transfection step (Figure 2). When used together with ReproTeSR™ reprogramming medium, the generation of iPS cell colonies can be achieved under feeder-free conditions with superior colony morphology and similar reprogramming efficiency to feeder-based systems (Figures 3 - 4). ReproRNA™-derived iPS cell colonies also express markers of undifferentiated cells and retain a normal karyotype (Figures 5-6). Subsequently, iPS cells generated with ReproRNA™-OKSGM can be maintained in TeSR™ maintenance media and further differentiated into cells of all three germ layers (Figure 7).
Apexbio Technology LLC PPACK Dihydrochloride 142036-63-3 5mg
PPACK Dihydrochloride is a small-molecule inhibitor targeting human -thrombin It is designed to inhibit thrombin activity thereby blocking downstream platelet aggregation and coagulation pathways PPACK Dihydrochloride exerts its biological activity primarily through selective irreversible inhibition of thrombin by covalently occupying the thrombin active-site serine residue and forming a stable tetrahedral intermediate with the catalytic His57 residue Based on these pharmacological properties PPACK Dihydrochloride holds research potential in biomedical studies as an anticoagulant reagent and for investigating thrombin-mediated coagulation and platelet activation mechanisms
Expression Systems BESTBAC 1.0 LINEAR BACULOVIRUS
Expression Systems’ popular BestBac Linearized Baculovirus DNA backbone has been engineered to deliver highly efficient recombination with all polyhedron gene locus based transfer vectors. The resulting virus vector provides superior stability and purity compared to other systems.
• Unmatched productivity and stability for BEVS
• Improved production methodology can reduce need for plaque purification
Produces high titers of 5 E8 to 3 E9 IU/ml of recombinant baculovirus in P1 stock
• Generates P1 stock as quickly as Bac-to-Bac® and other systems
• Each BestBac vial contains enough material for a minimum of five cotransfections
• Suitable for other expression systems including BacMam™
• BestBac 1.0 is wild type at the cathepsin and chitinase (v-cath/chiA) loci, preferred for some applications.
Included in the kit are:
• BestBac DNA
• pVL1393-lacZ control vector
• Transfection medium
• Expres2 TR transfection reagent